Molecular Neuro-Oncology workshop

7th Annual Conference of Neuropathology Society of India (NPSICON 2025) Organized By: Neuropathology Laboratory, AIIMS, New Delhi

WORKSHOP 1: METHYLATION PROFILING

Venue: JL Auditorium, AIIMS, New Delhi

Date: 20.02.2025

Workshop Coordinators: Drs. Kavneet Kaur and Aruna Nambirajan

Time Session Speaker/s Chairpersons
8:00 to 8:40 AM Registration
8:45 to 9:00 AM Welcome address Dr. MC Sharma
9:00 to 10:15 AM Basics of DNA methylation-based classification of CNS Tumors Dr. David Capper Dr. MC Sharma
Dr. Vani Santosh
Dr. Venkat Iyer
10:15 to 10:30 AM Discussion
10:30 to 11:00 AM (TEA BREAK)
11:00 AM to 12:30 PM Development of DNA methylation-based classifiers and data analysis Dr. Martin Sill Dr. BD Radotra
Dr. Geeta Chacko
Dr. Epari Sridhar
12:30 to 1:00 PM Discussion
1:00 to 2:00 PM (TEA BREAK)
2:00 to 3:00 PM MNP Outreach & Beyond – Nanopore based CNS tumour profiling Dr. Areeba Patel Dr. David Capper
Dr. Chitra Sarkar
Dr. Sunita Ahlawat
3:00 to 3:20 PM Potential, Promises and Pitfalls of Genomic Medicine Dr. Vedam Ramprasad
3:20 to 3:40 PM Infinium Methylation Array: A tool for high-resolution tumor characterization Dr. Ashutosh Upadhyay
3:40 to 4:00 PM Discussion
4:00 to 4:30 PM (TEA BREAK)
4:30 to 5:30 PM Interesting Case Studies Dr. Sushma Patil
Dr. Megha Uppin
Dr. Jyotsna
Dr. Kirti Gupta
Dr. Arpita Jindal
6:00 PM Onwards Inauguration and Social Evening

WORKSHOP 2: NEXT GENERATION SEQUENCING

Venue: SET Facility, AIIMS, New Delhi

Date: 20.02.2025

Workshop Coordinator: Dr. Saumyaranjan Mallick

Time Session Speaker/s Chairpersons
08:00 to 08:40 AM Registration
08:45 to 09:00 AM Welcome Address Dr. Saumyaranjan Mallick
09:00 to 09:15 AM Introduction to Next Generation Sequencing Dr. Saumyaranjan Mallick Dr. Nuzhat Hussain
Dr. Sonika Dahiya
Dr. Ritu Gupta
09:15 to 09:45 AM Library Preparation Dr. Nikhil Patkar
09:45 to 10:15 AM Quality control of Sample and Library Dr. Prerana Jha
10:15 to 10:45 AM Basics of Panel Designing Dr. Nikhil Patkar
10:45 to 11:00 AM Discussion
TEA BREAK (11:00 to 11:15 AM)
11:15 to 12:00 PM Panel Designing by Ampliseq Platform and Analysis of CGP Mr. Hersh Parikh
12:00 to 01:00 PM Laboratory Demonstration - Sequencing By Ion Torrent Platform Dr. Neeraj Chauhan
LUNCH (01:00 to 02:00 PM)
02:00 to 02:15 PM Newer Advances in Short Read Sequencer Dr. Pankaj Khanna Dr. Nikhil Patkar
Dr. Sudhir Arava
Dr. Saumyaranjan Mallick
02:15 to 02:45 PM Validation of NGS Assay and Bioinformatic Pipeline Dr. Suruchi Aggarwal
02:45 to 03:15 PM Variant Calling Dr. Moushumi Suryavanshi
03:15 to 03:30 PM Live Demonstration of NGS Variant Calling Dr. Saumyaranjan Mallick
03:30 to 03:45 PM Discussion
TEA BREAK (03:45 to 04:00 PM)
04:00 to 05:30 PM Interesting Case Presentations Dr. Anila Sharma
Dr. Kiranpreet Malhotra
Dr. Sunita Ahlawat
Dr. Priti Trivedi
Dr. Meghna Chougle
Dr. Sonika Dahiya
Dr. Anila Sharma
Dr. Sudeep Khera
06:00 PM Onwards Inauguration and Social Evening

WORKSHOP 3: SANGER SEQUENCING, FRAGMENT ANALYSIS AND DIGITAL PCR

Venue: SET facility, AIIMS, New Delhi

Date: 20.02.2025

Workshop Coordinator: Dr. Anubhav Narwal

Time Session Speaker/s Chairpersons
08:00 to 08:40 AM Registration
08:45 to 09:00 AM Welcome Address Dr. Anubhav Narwal
09:00 to 09:30 AM Introduction to Sanger Sequencing Dr. Deepshi Thakral Dr. Kunzang Chosdal
Dr. Prasanjit Das
Dr. Asit Ranjan Mirdha
09:30 to 10:00 AM Basics of Fragment Analysis and Laboratory Methodology Dr. Anil Kumar
10:00 to 10:45 AM Primer Designing and Sequencing Method Dr. Neeraj Arora
10:45 to 11:00 AM Discussion
TEA BREAK (11:00 to 11:15 AM)
11:15 to 12:00 PM Live demonstration of Analysis of Sanger Sequencing Data Dr. Anil Kumar
12:00 to 12:20 PM T & B cell clonality assay by Fragment Analysis Dr. Saumyaranjan Mallick
12:20 to 01:00 PM Laboratory demonstration of Equipment and Consumable Dr. Anil Kumar and Team
LUNCH (01:00 to 02:00 PM)
02:00 to 02:30 PM Basics of Digital PCR Dr. Prashant Sharma Dr. Deepali Jain
Dr. Shipra Agarwal
Dr. Ashwani Tandon
02:30 to 03:00 PM Lab Methodology For Digital Droplet PCR Dr. Suvendu Purkait
03:00 to 03:30 PM Data Analysis of Digital PCR Dr. Prashant Sharma
03:30 to 03:45 PM Discussion
TEA BREAK (03:45 to 04:00 PM)
04:00 to 04:30 PM Demonstration DD PCR Equipment Company
04:30 to 05:30 PM Interesting Case Presentation Dr. Gaurav Khanna
Dr. Swati Singh
Dr. Saumya Sahu
Dr. Vineeta Batra
Dr. Vandita Singh
Dr. Manu Kukreja
06:00 PM Onwards Inauguration and Social Evening

WORKSHOP 4: FISH ASSAY

Venue: Conference Hall, AIIMS, New Delhi

Date: 20.02.2025

Workshop Coordinator: Dr. Balamurugan T

Time Session Speaker/s Chairpersons
08:00 to 08:40 AM Registration
08:45 to 09:00 AM Welcome Address Dr. Balamurugan T
09:00 to 09:25 AM Principles of Fluorescence In Situ Hybridization Dr. Poonam Elhence Dr. Catriona McLean
Dr. Poonam Elhence
Dr. Sunila Jain
09:25 to 09:50 AM Overview of FISH Probes and Target Regions Dr. Rakesh Gupta
09:50 to 10:15 AM Applications of FISH in CNS Tumor Diagnosis Dr. Swati Mahajan
10:15 to 10:30 AM Discussion
TEA BREAK (10:30 to 11:00 AM)
11:00 to 11:30 AM Advancement in FISH M/S Invitro Lab Services Dr. Catriona McLean
Dr. Arvind Ahuja
11:30 to 01:00 PM Hands-on experience with tissue sample preparation and slide preparation for FISH assay Company Dr. Swati Mahajan
Dr. Hemlata
LUNCH (01:00 to 02:00 PM)
02:00 to 03:00 PM Hands-on experience with the hybridization process, probe application, and detection of FISH signals Company Dr. Swati Mahajan
Dr. Hemlata
03:00 to 04:00 PM Analyzing FISH Results: Spot Counting and Signal Interpretation
TEA BREAK (04:00 to 04:30 PM)
04:30 to 05:30 PM Interesting Case Presentations Dr. Sushila Jaiswal
Dr. Meenakshi Swain
Dr. Supriya Menon
Dr. Fouzia Siraj
Dr. Surender Kumar Verma
Dr. Yookarin Khonglah
Dr. Mukund Sable
06:00 PM Onwards Inauguration and Social Evening