7th Annual Conference of Neuropathology Society of India (NPSICON 2025) Organized By: Neuropathology Laboratory, AIIMS, New Delhi
WORKSHOP 1: METHYLATION PROFILING
Venue: JL Auditorium, AIIMS, New Delhi
Date: 20.02.2025
Workshop Coordinators: Drs. Kavneet Kaur and Aruna Nambirajan
| Time | Session | Speaker/s | Chairpersons |
|---|---|---|---|
| 8:00 to 8:40 AM | Registration | ||
| 8:45 to 9:00 AM | Welcome address | Dr. MC Sharma | |
| 9:00 to 10:15 AM | Basics of DNA methylation-based classification of CNS Tumors | Dr. David Capper | Dr. MC Sharma Dr. Vani Santosh Dr. Venkateswaran K. Iyer |
| 10:15 to 10:30 AM | Discussion | ||
| 10:30 to 11:00 AM (TEA BREAK) | |||
| 11:00 AM to 12:30 PM | DNA Methylation: Methodology and Data Assessment | Dr. Jyotsna Singh | Dr. BD Radotra Dr. Geeta Chacko Dr. Epari Sridhar |
| 12:30 to 1:00 PM | Discussion | ||
| 1:00 to 2:00 PM (LUNCH BREAK) | |||
| 2:00 to 2:45 PM | MNP Outreach & Beyond – Nanopore based CNS tumour profiling | Dr. Areeba Patel | Dr. David Capper Dr. Chitra Sarkar Dr. Sunita Ahlawat |
| 2:45 to 3:00 PM | Infinium Methylation Array : A tool for high-resolution tumour characterization | Dr. Ashutosh Upadhyay | |
| 3:00 to 3:15 PM | Methylation-based Clinical Diagnostics – MedGenome's Perspective | Dr. Ravi Gupta | |
| 3:15 to 3:30 PM | Discussion | ||
| 3:30 to 4:30 PM | Interesting Case Studies | Dr. Jyotsna Dr. Megha Uppin Dr. Sushma Patil |
Dr. Kirti Gupta Dr. Arpita Jindal |
| 4:30 PM (TEA BREAK) | |||
| 5:00 PM Onwards | Inauguration and Social Evening | ||
WORKSHOP 2: NEXT GENERATION SEQUENCING
Venue: SET Facility, AIIMS, New Delhi
Date: 20.02.2025
Workshop Coordinator: Dr. Saumyaranjan Mallick
| Time | Session | Speaker/s | Chairpersons |
|---|---|---|---|
| 08:00 to 08:40 AM | Registration | ||
| 08:45 to 09:00 AM | Welcome Address | Dr. Saumyaranjan Mallick | |
| 09:00 to 09:15 AM | Introduction to Next Generation Sequencing | Dr. Saumyaranjan Mallick | Dr. Nuzhat Hussain Dr. Sonika Dahiya Dr. Ritu Gupta |
| 09:15 to 09:45 AM | Library Preparation | Dr. Nikhil Patkar | |
| 09:45 to 10:15 AM | Quality control of Sample and Library | Dr. Prerana Jha | |
| 10:15 to 10:45 AM | Basics of Panel Designing | Dr. Nikhil Patkar | |
| 10:45 to 11:00 AM | Discussion | ||
| TEA BREAK (11:00 to 11:15 AM) | |||
| 11:15 to 12:00 PM | Panel Designing by Ampliseq Platform and Analysis of CGP | Mr. Hersh Parikh | |
| 12:00 to 01:00 PM | Laboratory Demonstration - Sequencing By Ion Torrent Platform | Dr. Neeraj Chauhan | |
| LUNCH (01:00 to 02:00 PM) | |||
| 2:00 to 2:30 PM | Validation of NGS Assay and Bioinformatic Pipeline | Dr. Suruchi Aggarwal | Dr. Nikhil Patkar Dr. Sudhir Arava Dr. Saumyaranjan Mallick |
| 2:30 to 2:45 PM | Newer advances in short read sequencer | Dr. Priyadarshini Pande | |
| 2:45 to 3:15 PM | Variant Calling | Dr. Moushumi Suryavanshi | |
| 3:15 to 3:30 PM | Live demonstration of NGS Variant Calling | Dr Saumyaranjan Mallick | |
| 3:30 to 3:45 PM | Discussion | ||
| 3:45 to 4:30 PM | Interesting case presentation | Dr.Anila Sharma Dr.Kiranpreet Malhotra Dr.Sunita Ahlawat Dr.Meghna Chougle |
Dr. SonikaDahiya Dr.Anila Sharma Dr. SudeepKhera |
| 4:30 PM (TEA BREAK) | |||
| 5:00 PM Onwards | Discussion | ||
WORKSHOP 3: SANGER SEQUENCING, FRAGMENT ANALYSIS AND DIGITAL PCR
Venue: SET facility, AIIMS, New Delhi
Date: 20.02.2025
Workshop Coordinator: Dr. Anubhav Narwal
| Time | Session | Speaker/s | Chairpersons |
|---|---|---|---|
| 08:00 to 08:40 AM | Registration | ||
| 08:45 to 09:00 AM | Welcome Address | Dr. Anubhav Narwal | |
| 09:00 to 09:30 AM | Introduction to Sanger Sequencing | Dr. Deepshi Thakral | Dr. Kunzang Chosdal Dr. Prasanjit Das Dr. Asit Ranjan Mirdha |
| 09:30 to 10:00 AM | Basics of Fragment Analysis and Laboratory Methodology | Dr. Anil Kumar | |
| 10:00 to 10:45 AM | Primer Designing and Sequencing Method | Dr. Spandan Chaudhry | |
| 10:45 to 11:00 AM | Discussion | ||
| TEA BREAK (11:00 to 11:15 AM) | |||
| 11:15 to 12:00 PM | Live demonstration of Analysis of Sanger Sequencing Data | Dr. Anil Kumar | |
| 12:00 to 12:20 PM | T & B cell clonality assay by Fragment Analysis | Dr. Saumyaranjan Mallick | |
| 12:20 to 01:00 PM | Laboratory demonstration of Equipment and Consumable | Dr. Anil Kumar and Team | |
| LUNCH (01:00 to 02:00 PM) | |||
| 2:00 to 2:20 PM | Basics of Digital PCR | Dr. Prashant Sharma | Dr. Deepali Jain Dr. Shipra Agarwal Dr. Ashwani Tandon |
| 2:20 to 2:40 PM | Lab Methodology For Digital Droplet PCR | Dr. Suvendu Purkait | |
| 2:40 to 3:00 PM | Data Analysis of Digital PCR | Dr. Prashant Sharma | |
| 3:00 to 3:15 PM | Discussion | ||
| 3:15 to 4:00 PM | Interesting case presentations | Dr.Gaurav Khanna Dr.Swati Singh Dr.Saumya Sahu |
Dr.Vineeta Batra Dr.Vandita Singh Dr. Manu Kukreja |
| 4:00 to 4:30 PM | Demonstration DD PCR Equipment | Company | |
| TEA BREAK ( 04:30 PM) | |||
| 5:00 PM Onwards | Inauguration and Social Evening | ||
WORKSHOP 4: FISH ASSAY
Venue: Conference Hall, AIIMS, New Delhi
Date: 20.02.2025
Workshop Coordinator: Dr. Balamurugan T
| Time | Session | Speaker/s | Chairpersons |
|---|---|---|---|
| 08:00 to 08:40 AM | Registration | ||
| 08:45 to 09:00 AM | Welcome Address | Dr. Balamurugan T | |
| 09:00 to 09:25 AM | Principles of Fluorescence In Situ Hybridization | Dr. Poonam Elhence | Dr. Catriona McLean Dr. Poonam Elhence Dr. Sunila Jain |
| 09:25 to 09:50 AM | Overview of FISH Probes and Target Regions | Dr. Rakesh Gupta | |
| 09:50 to 10:15 AM | Applications of FISH in CNS Tumor Diagnosis | Dr. Swati Mahajan | |
| 10:15 to 10:30 AM | Discussion | ||
| TEA BREAK (10:30 to 11:00 AM) | |||
| 11:00 to 11:30 AM | Advancement in FISH | Dr Shweta | Dr. Catriona McLean Dr. Arvind Ahuja |
| 11:30 to 01:00 PM | Hands-on experience with tissue sample preparation and slide preparation for FISH assay | Company | Dr. Swati Mahajan Dr. Hemlata |
| LUNCH (01:00 to 02:00 PM) | |||
| 2:00 to 3:30 pm | Hands-on experience with the hybridization process, probe application, and detection of FISH signals Analyzing FISH Results: Spot Counting and Signal Interpretation |
Company | Dr. Swati Mahajan Dr. Hemlata |
| 3:30 to 4:30 PM | Interesting Case Presentations | Dr. Sushila Jaiswal Dr. Meenakshi Swain Dr. Supriya Menon Dr. Fouzia Siraj |
Dr.Surender Kumar Verma Dr.YookarinKhonglah Dr.Mukund Sable |
| TEA BREAK (4:30 PM) | |||
| 5:00 PM Onwards | Inauguration and Social Evening | ||